Highlights

Electroporation-Based Technologies and Treatments (EBTT)
Postgraduate Course and Scientific Workshop
Ljubljana, November 10—16 2019.
13th Edition
http://2019.ebtt.org/

 

 

Nominations
 2018

International Society for Electroporation-Based Technologies and Treatments

President elect:
Luis Mir
Officers: Biological sciences:
Marie-Pierre Rols; Maja Čemažar



 

 

Recent Publications


2018

Papers published within the scope of the LEA

Batista Napotnik T, Miklavčič D. In vitro electroporation detection methods – An overview. Bioelectrochemistry 120: 166-182, 2018. DOI 10.1016/j.bioelechem.2017.12.005

Boc N, Edhemović I, Kos B, Marolt-Mušič M, Brecelj E, Trotovšek B, Bošnjak M, Djokić M, Miklavčič D, Čemažar M, Serša G. Ultrasonographic changes in the liver tumors as indicators of adequate tumor coverage with electric field for effective electrochemotherapy. Radiol. Oncol. 52: 383-391, 2018. DOI 10.2478/raon-2018-0041

Cindrič H, Kos B, Tedesco G, Cadossi M, Gasbarrini A, Miklavčič D. Electrochemotherapy of spinal metastases using transpedicular approach – A numerical feasibility study. Technol. Cancer Res. Treat. 17: 1-13, 2018. DOI 10.1177/153303461877025

Dermol-Černe J, Vidmar J, Ščančar J, Uršič K, Serša G, Miklavčič D. Connecting the in vitro and in vivo experiments in electrochemotherapy – a feasibility study modeling cisplatin transport in mouse melanoma using the dual-porosity model. J. Control. Release 286: 33-45, 2018. DOI 10.1016/j.jconrel.2018.07.021

Djokić M, Čemažar M, Popovič P, Kos B, Dežman R, Bošnjak M, Nikšić Žakelj M, Miklavčič D, Potrč S, Štabuc B, Tomažič A, Serša G, Trotovšek B. Electrochemotherapy as treatment option for hepatocellular carcinoma, a prospective pilot study. Eur. J. Surg. Oncol. 44: 651-657, 2018. DOI 10.1016/j.ejso.2018.01.090

Forjanič T, Miklavčič D. Numerical study of gene electrotransfer efficiency based on electroporation volume and electrophoretic movement of plasmid DNA. Biomed. Eng. Online 17:80: 1-10, 2018. DOI 10.1186/s12938-018-0515-3

Kulbacka J, Paczuska J, Rembiałkowska N, Saczko J, Kiełbowicz Z, Kinda W, Liszka B, Kotulska M, Kos B, Miklavčič D, Tozon N, Čemažar M. Electrochemotherapy combined with standard and CO2 laser surgeries in canine oral melanoma. Slov. Vet. Res. 54(4): 181-186, 2018. DOI 10.26873/SVR-322-2017

Pintar M, Langus J, Edhemović I, Brecelj E, Kranjc M, Serša G, Šuštar T, Rodič T, Miklavčič D, Kotnik T, Kos B. Time-dependent finite-element analysis of in vivo electrochemotherapy treatment. Technol. Cancer. Res. Treat. 17: 1-9, 2018. DOI 10.1177/1533033818790510

Pirc E, Pecchia L, Reberšek M, Serša G, Snoj M, Grošelj A, Miklavčič D. Study design of a medical device pre-market assessment: a case study on electrochemotherapy. Zdrav. Vestn. 87: 22-40, 2018. DOI 10.6016/ZdravVestn.2482

Savarin M, Prevc A, Rzek M, Bošnjak M, Vojvodić I, Čemažar M, Jarm T, Serša G. Intravital monitoring of vasculature after targeted gene therapy alone or combined with tumor irradiation. Technol. Cancer Res. Treat. 17: 1-8, 2018. DOI 10.1177/153303381878420

Vera-Tizatl AL, Vera-Tizatl CE, Vera-Hernandez A, Leija-Salas L, Rodriguez S, Miklavčič D, Kos B. Computational feasibility analysis of electrochemotherapy with novel needle-electrode arrays for the treatment of invasive breast ductal carcinoma. Technol. Cancer Res. Treat. 17: 1-13, 2018. DOI 10.1177/1533033818794939

Deville, R. Natalini, C. Poignard. A Continuum Mechanics Model of Enzyme-Based Tissue Degradation in Cancer Therapies.   Bull. Math. Biol., (2018).

Michel, J. Fehrenbach, V. Lobjois, J. Laurent, A. Gomes, T. Colin, C. Poignard. Mathematical modeling of the proliferation gradient in multicellular tumor spheroids. Journal of Theoretical Biology, 458, 133-147, 2018.

D.Voyer, A. Silve, L.M. Mir, R. Scorretti, C. Poignard. Dynamical modeling of tissue electroporation. Bioelectrochemistry, 119, 98-110, 2018.

Pasquet, L. Bellard, E., Rols, MP., Golzio, M., Teissié, J. 2018. Non-invasive gene electrotransfer in skin. Human Gene Therapy Methods (in Press).

Kolosnjaj-Tabi, J., Gibot, L., Golzio, M and Rols, MP. Electric field-responsive nanoparticles and the electric field: physical, chemical, and biological aspects for therapy and diagnosis enhancement. Advanced Drug Delivery Reviews. 2018

 De Robertis, M., L. Pasquet, L. Loiacono, E. Bellard, L. Messina, S. Vaccaro, R. Di Pasquale, V. M. Fazio, M. P. Rols, J. Teissie, M. Golzio*, and E. Signori*. In Vivo Evaluation of a New Recombinant Hyaluronidase to Improve Gene Electro-Transfer Protocols for DNA-Based Drug Delivery against Cancer. Cancers 10, 2018

García-Sánchez, A. Muscat, I. Leray and L. M. Mir. Pyroelectricity as a possible mechanism for cell membrane permeabilization. Bioelectrochemistry 119 (2018) 227–233; doi:10.1016/j.bioelechem.2017.10.007

García-Sánchez, C. Merla, J. Fontaine, A. Muscat, L. M. Mir. Sine wave electropermeabilization reveals the frequency-dependent response of the biological membranes. Biochemical Biophysical Acta – Biomembranes, 1860, 1022-1034, 2018.

Merla, M. Liberti, P. Marracino, A. Muscat, A. Azan, F. Apollonio, L. M. Mir. A wide-band bio-chip for real-time optical detection of bioelectromagnetic interactions with cells. Sci. Rep. (2018) 8:5044 doi.org/10.1038/s41598-018-23301-w

García-Sánchez, R. Bragós, L. M. Mir. In vitro analysis of various cell lines responses to electroporative electric pulses by means of electrical impedance spectroscopy. Biosensors and Bioelectronics, 117: 207-216, 2018

Hanna, L. M. Mir, F. M. Andre. In vitro osteoblastic differentiation of mesenchymal stem cells generates cell layers with distinct properties. Stem Cells Research and Therapy, 9:203 (11 pages), 2018 (open access)

 Teissié J. Induced shock waves in PEF (pulsed electric field) treatment: Comment on "Shock wave-induced permeabilization of mammalian cells" by Luz M. López-Marín et al. Phys Life Rev. 2018 May 17. pii: S1571-0645(18)30062-9. doi: 10.1016/j.plrev.2018.05.004

URŠIČ, Katja, KOS, Špela, KAMENŠEK, Urška, ČEMAŽAR, Maja, ŠČANČAR, Janez, BUČEK, Simon, KRANJC BREZAR, Simona, STAREŠINIČ, Barbara, SERŠA, Gregor. Comparable effectiveness and immunomodulatory actions of oxaliplatin and cisplatin in electrochemotherapy of murine melanoma. Bioelectrochemistry, ISSN 1567-5394. [Print ed.], Feb. 2018, vol. 119, p. 161-171, doi: 10.1016/j.bioelechem.2017.09.009.

LAMPREHT TRATAR, Urša, KOS, Špela, KAMENŠEK, Urška, OTA, Maja, TOZON, Nataša, SERŠA, Gregor, ČEMAŽAR, Maja. Antitumor effect of antibiotic resistance gene-free plasmids encoding interleukin-12 in canine melanoma model. Cancer gene therapy, ISSN 0929-1903. [Print ed.], 2018, vol. , no. , p. [1-10], doi: 10.1038/s41417-018-0014-5

KAMENŠEK, Urška, ČEMAŽAR, Maja, LAMPREHT TRATAR, Urša, URŠIČ, Katja, SERŠA, Gregor. Antitumor in situ vaccination effect of TNF[alfa] and IL-12 plasmid DNA electrotransfer in a murine melanoma model. Cancer immunology and immunotherapy, ISSN 0340-7004, 2018, vol. 67, no. 5, p. 785-795, doi: 10.1007/s00262-018-2133-0.

KAMENŠEK, Urška, TEŠIĆ, Nataša, SERŠA, Gregor, ČEMAŽAR, Maja. Clinically usable interleukin 12 plasmid without an antibiotic resistance gene : functionality and toxicity study in murine melanoma model. Cancers, ISSN 2072-6694, 2018, vol. 10, no. 3, p. [1-14]. doi: 10.3390/cancers10030060.

BOŠNJAK, Maša, KAMENŠEK, Urška, SERŠA, Gregor, ŠTOLFA, Danijela, LAVRENČAK, Jaka, ČEMAŽAR, Maja. Inhibition of the innate immune receptors for foreign DNA sensing improves transfection efficiency of gene electrotransfer in melanoma B16F10 cells. The journal of membrane biology, ISSN 0022-2631, 2018, vol. 251, iss. 2, p. 179-185, doi: 10.1007/s00232-017-9948-z.

 STAREŠINIČ, Barbara, JESENKO, Tanja, KAMENŠEK, Urška, KROG FRANDSEN, Stine, SERŠA, Gregor, GEHL, Julie, ČEMAŽAR, Maja. Effect of calcium electroporation on tumour vasculature. Scientific reports, ISSN 2045-2322, 2018, vol. 8, no. 1, p. 1-8. doi: 10.1038/s41598-018-27728-z.

 Co-authored papers between French and Sloven partners

Kotnik, L. Rems, M. Tarek and D. Miklavčič. Membrane Electroporation and Electropermeabilization: Mechanisms and Models. Annu. Rev. Biophys. 2019; 48:X–X https://doi.org/10.1146/annurev-biophys-052118-115451

Markelc B, Bellard E, Sersa G, Jesenko T, Pelofy S, Teissié J, Frangez R, Rols MP, Cemazar M, Golzio M. Increased permeability of blood vessels after reversible electroporation is facilitated by alterations in endothelial cell-to-cell junctions. J Control Release. 2018;276:30-41. doi: 10.1016/j.jconrel.2018.02.032

Teissié J, Miklavcic D. Special issue on bacterial inactivation. Bioelectrochemistry. 2018 Oct;123:260. doi: 10.1016/j.bioelechem.2017.01.001

Rems L, Viano M, Kasimova MA, Miklavčič D, Tarek M. The contribution of lipid peroxidation to membrane permeability in electropermeabilization: A molecular dynamics study. Bioelectrochemistry 125: 46-57, 2019. DOI 10.1016/j.bioelechem.2018.07.018

Čemažar M, Serša G, Frey W, Miklavčič D, Teissié J. Recommendations and requirements for reporting on applications of electric pulse delivery for electroporation of biological samples. Bioelectrochemistry 122: 69-76, 2018. DOI 10.1016/j.bioelechem.2018.03.005

Dermol-Černe J, Miklavčič D, Reberšek M, Mekuč P, Bardet SM, Burke R, Arnaud-Cormos D, Leveque P, O'Connor R. Plasma membrane depolarization and permeabilization due to electric pulses in cell lines of different excitability. Bioelectrochemistry 122: 103-114, 2018. DOI 10.1016/j.bioelechem.2018.03.011

Forjanič T, Markelc B, Marčan M, Bellard E, Couillaud F, Golzio M, Miklavčič D. Electroporation induced stress response and its effect on gene electrotransfer efficacy: in vivo imaging and numerical modelling. Submitted to IEEE Transactions on Biomedical Engineering.

Pasquet L, Chabot S, Bellard E, Markelc B, Rols, M-P., Reynes J-P, Tiraby G, Couillaud F, Teissié J, Golzio M. Safe and efficient novel approach for non-invasive gene electrotransfer to skin. Scientific reports, ISSN 2045-2322, 2018, vol. 8, no. 1. https://www.nature.com/articles/s41598-018-34968-6, doi: 10.1038/s41598-018-34968-6.

 

2017

Papers published within the scope of the LEA

Denzi, E. della Valle, F. Apollonio, M. Breton, L. M. Mir and M. Liberti. Exploring the Applicability of Nano-poration for Remote Control in Smart Drug Delivery Systems. J. Membrane Biol, 250, 31-40, 2017.

Azan, V. Untereiner, C. Gobinet, G. D. Sockalingum, M. Breton, O. Piot and L. M. Mir. Demonstration of Protein Involvement in Living Cell Electropulsation using Confocal Raman Microspectroscopy. Scientific Reports 7. 297–306, 2017.

Azan, P.J. Caspers, T.C. Bakker Schut, S. Roy, C. Boutros, C. Mateus, E. Routier, B. Besse, D. Planchard, A. Seck, N. Kamsu Kom, G. Tomasic, S. Koljenović, V. Noordhoek Hegt, A. Celebic, M. Texier, E. Lanoy, A.M.M. Eggermont, A. Paci, C. Robert, G.J. Puppels, L.M. Mir. A Novel Spectroscopically Determined Pharmacodynamic Biomarker for Skin Toxicity in Cancer Patients Treated with Targeted Agents. Cancer Res. 77: 557-565, 2017

Sanz, I. Leray, A. Dewaele, D. Grébert, S. Antoine, A. Acquistapace, A Muscat, A Boukadiri and L. M. Mir. Structurally Related Odorant Ligands of the Olfactory Receptor OR51E2 Differentially Promote Metastasis Emergence and Tumor Growth. Oncotarget, 8, 4330-4341, 2017

Denzi, E. della Valle, G. Esposito, L. M. Mir, F. Apollonio and M. Liberti. Technological and Theoretical Aspects for Testing Electroporation on Liposomes. BioMed Research International, vol. 2017, Article ID 5092704, 2017

Hanna, F.M. Andre and L.M. Mir. Electrical control of calcium oscillations in mesenchymal stem cells using microsecond pulsed electric fields. Stem cell research and therapy, vol 8, art 91, 2017

D.Voyer, A. Silve, L. M. Mir, R. Scorretti and C. Poignard. Dynamic modeling of tissue electroporation. Bioelectrochemistry 119: 98-110 (2018)

Breton and L. M. Mir. Investigation of the Chemical Mechanisms Involved in the Electropulsation of Membranes at the Molecular Level. Bioelectrochemistry 119 (2018) e7966;

Azan, V. Untereiner, L. Descamps, C. Merla, C. Gobinet, M. Breton, O. Piot and L. M. Mir. Comprehensive Characterization of the Interaction between Pulsed Electric Fields and Live Cells by Confocal Raman Microspectroscopy. Analytical Chemistry 89, 10790-10797, 2017.

Hanna, A. Denzi, M. Liberti, F.M. Andre and L.M. Mir. Electropermeabilization of inner and outer membranes of cells with microsecond pulsed electric fields: Quantitative study with calcium ions. Sci. Rep. 7, art. 13079, 2017

García-Sánchez, A. Muscat, I. Leray and L. M. Mir. Pyroelectricity as a possible mechanism for cell membrane permeabilization. Bioelectrochemistry 119 (2018) 227–233;

A. Ascierto, S. Agarwala, G. Ciliberto, S. Demaria, R. Dummer, C. Duong, S. Ferrone, S. C. Formenti, C. Garbe, R. Halaban, S. Khleif, J. Luke, L. M. Mir, W. W. Overwijk, M. Postow, I. Puzanov, P. Sondel, J. M. Taube, P. T. Straten, D. F. Stroncek, J. A. Wargo, H. Zarour, and M. Thurin Future perspectives in melanoma research - “Melanoma Bridge”, Napoli, November 30th- 3rd December 2016. J. Transl. Med. 15:236-267 2017

Pillet, F., L. Gibot, M. Madi, M. P. Rols, and E. Dague. 2017. Importance of endogenous extracellular matrix in biomechanical properties of human skin model. Biofabrication 9:025017.

Tamra, A., D. Dubuc, M. P. Rols, and K. Grenier. 2017. Microwave Monitoring of Single Cell Monocytes Subjected to Electroporation. IEEE Transactions on Microwave Theory and Techniques 99:1-7.

Batista Napotnik T., Bello G., Sinner E.K., Miklavčič D. The Effect of Nanosecond, High-Voltage Electric Pulses on the Shape and Permeability of Polymersome GUVs. J Membr Biol 250: 441-453, 2017

Dermol-Černe J., Miklavčič D. From Cell to Tissue Properties – Modeling Skin Electroporation With Pore and Local Transport Region Formation. IEEE T. Biomed. Eng. PP/99: 1-11, 2017

Garcia P.A., Kos B., Rossmeisl J.H.Jr., Pavliha D., Miklavčič D.. Predictive therapeutic planning for irreversible electroporation treatment of spontaneous malignant glioma. Med Phys 44: 4968-4980, 2017

Kranjc M., Kranjc S., Bajd F., Serša G., Serša I., Miklavčič D. Predicting irreversible electroporation-induced tissue damage by means of magnetic resonance electrical impedance tomography. Sci. Rep. 7: 10323: 1-10, 2017

Mahnič-Kalamiza S., Poklar Ulrih N., Vorobiev E., Miklavčič D. A comprehensive theoretical study of thermal relations in plant tissue following electroporation. Int J Heat Mass Transfer: 150-162, 2017

Novickij V., Dermol J., Grainys A., Kranjc M., Miklavčič D. Membrane permeabilization of mammalian cells using bursts of high magnetic field pulses. PeerJ 5:e3267

Burke R., Bardet S.M., Carr L., Romanenko S., Arnaud-Cormos D., Leveque P., O’Connor R.P. “Nanosecond pulsed electric fields depolarize transmembrane potential via voltage-gated K+, Ca2+ and TRP channels”. Biochimica et biophysica acta, 1859(10), pp. 2040-2050. Published: Oct. 2017

Carr L., Bardet S.M., Arnaud-Cormos D., Leveque P., O’Connor R.P., “Visualisation of an nsPEF induced calcium wave using the genetically encoded calcium indicator GCaMP in U87 human glioblastoma cells”. Bioelectrochemistry, 119C, 68-75 (2018).

Soueid M., Dobbelaar M.C.F., Bentouati S., Bardet S.M., O’Connor R.P., Bessieres D., Paillol J., Leveque P., Arnaud-Cormos D., “Delivery devices for exposure of biological cells to nanosecond pulsed electric fields”. Medical & Biological Engineering & Computing, pp.1-13. Published: 4 Jul. 2017.

Carr L., Bardet S.M., Arnaud-Cormos D., Leveque P., O’Connor R.P.“Calcium-independent disruption of microtubule dynamics by nanosecond pulsed electric fields in U87 human glioblastoma cells”. Scientific Report, 7, 41267. 2017. 

Kamensek U, Tesic N, Sersa G, Kos S, Cemazar M. Tailor-made fibroblast-specific and antibiotic-free interleukin 12 plasmid for gene electrotransfer mediated cancer immunotherapy. Plasmid 2017; 89: 9-15

Kos S, Vanvarenbergb K, Dolinsek T, Cemazar M, Jelenc J, Préat V, Sersa G, Vandermeulen G. Gene electrotransfer into skin using noninvasive multi-electrode array for vaccination and wound healing. Bioelectrochemistry 2017; 114: 33-41.

Savarin M, Kamensek U, Cemazar M, Heller R, Sersa G. Electrotransfer of plasmid DNA radiosensitizes B16F10 tumors through activation of immune response. Radiol Oncol 2017; 51: 30-9

Bosnjak M, Kamensek U, Stolfa D, Lavrencak J, Sersa G, Cemazar M. Inhibition of the innate immune receptors for foreign DNA sensing improves transfection efficiency of gene electrotransfer in melanoma B16F10 cells. J Membrane Biol 2017

Kos S, Blagus T, Cemazar M, Filipic G, Sersa G, Cvelbar U. Safety aspects of atmospheric pressure helium plasma jet operation on skin: in vivo study on mouse skin. Plos One 2017; 12(4): e0174966

Gasljevic G, Edhemovic I, Cemazar M, Brecelj E, Gadzijev EM, Music MM, Sersa G. Histopathological findings in colorectal liver metastases after electrochemotherapy. Plos One 2017; 12(7): e0180709

Kranjc M, Kranjc S, Bajd F, Sersa I, Sersa G. Miklavcic D. Predicting irreversible electroporation-induced tissue damage by means of magnetic resonance electrical impedance tomography. Sci Rep 2017; 7(1): 10323

Salvadori C, Svara T, Rocchigiani G, Millanta F, Pavlin D, Cemazar M, Lampreht Tratar U, Sersa G, Tozon N, Poli A. Effects of electrochemotherapy with cisplatin and peritumoral IL-12 gene electrotransfer on canine mast cell tumors: a histopathologic and immunohistochemical study. Radiol Oncol 2017; 51: 286-94. 2017

Ursic K, Kos S, Kamensek U, Cemazar M, Scancar J, Bucek S, Kranjc S, Staresinic B, Sersa G. Comparable effectiveness and immunomodulatory actions of oxaliplatin and cisplatin in electrochemotherapy of murine melanoma. Bioelectrochemistry 2017; 119: 161-171.

 Co-authored papers between French and Sloven partners

Casciola M, Kasimova MA, Rems L, Zullino S, Apollonio F, Tarek M. Properties of lipid electropores I: Molecular dynamics simulations of stabilized pores by constant charge imbalance. Bioelectrochemistry 109: 108-116, 2016.

Pillet F, Marjanovič I, Reberšek M, Miklavčič D, Rols MP, Kotnik T. Inactivation of spores by electric arcs. BMC Microbiol. 16: 148, 2016.

Raso J, Frey W, Ferrari G, Pataro G, Knorr D, Teissié J, Miklavčič D. Recommendations guidelines on the key information to be reported in studies of application of PEF technology in food and biotechnological processes. Innov. Food Sci. Emerg. Technol. 37: 312-321, 2016.

Rems L, Tarek M, Casciola M, Miklavčič D. Properties of lipid electropores II: Comparison of continuum-level modeling of pore conductance to molecular dynamics simulations. Bioelectrochemistry 112: 112-124, 2016. 

Rosazza C, Haberl Meglič S, Zumbusch A, Rols MP, Miklavčič D. Gene electrotransfer: a mechanistic perspective. Curr. Gene Ther. 16: 98-129, 2016.

Miklavcic, L.M. Mir and P. T. Vernier. Foreword to Sixth Special Issue on Electroporation-Based Technologies and Treatments. J Membrane Biol 249:591, 2016

Tozon N, Lampreht Tratar U, Žnidar K, Sersa G, Teissié J, Čemazar M. Operating procedures of the electrochemotherapy for treatment of tumor in dogs and cats. Journal of visualized experiments, ISSN 1940-087X, Oct. 2016, iss. 116. http://www.jove.com/video/54760/operating-procedures-electrochemotherapy-for-treatment-tumor-dogs, doi: 10.3791/54760.

Kamensek U, Rols M-P, Čemazar M, Golzio M. Visualization of nonspecific antitumor effectiveness and vascular effects of gene electro-transfer to tumors. Current gene therapy, 2016, vol. 16, 90-97.

 

2016

Papers published within the scope of the LEA

 

Silve, I. Leray, C. Poignard and L M. Mir. Impact of external medium conductivity on cell membrane electropermeabilization by microsecond and nanosecond electric pulses. Scientific Reports, Volume: 6, Article Number: 19957, Published: FEB 1 2016

L.L. Lesueur, L.M. Mir and F.M. André. Overcoming the specific toxicity of large plasmids electrotransfer in primary cells in vitro. Molecular Therapy - Nucleic Acids, Vol. 5, article number e291, Published March 8 2016; doi:10.1038/mtna.2016.4

  1. Calvet and L. M. Mir. The promising alliance of anticancer electrochemotherapy with immunotherapy. Cancer Metastases Reviews, 35(2), 165-177, 2016 DOI: 10.1007/s10555-016-9615-3

M.-A. de Menorval, F. Andre, A. Silve, C. Dalmay, O. Français, B. Le Pioufle, and L. M. Mir. Electric pulses: a flexible tool to manipulate cytosolic calcium concentrations and generate spontaneous-like calcium oscillations in mesenchymal stem cells. Sci. Rep. 6, 32331; doi: 10.1038/srep32331 (2016).

Tamošiūnas, L.M. Mir, W-S. Chen, A. Lihachev, M. Venslauskas and S. Šatkauskas. Intracellular delivery of bleomycin by combined application of electroporation and sonoporation in vitro. J Membrane Biol, 249: 677-700, 2016.

Denzi, E. della Valle, F. Apollonio, M. Breton, L. M. Mir and M. Liberti. Exploring the Applicability of Nano-poration for Remote Control in Smart Drug Delivery Systems. J Membrane Biol, DOI 10.1007/s00232-016-9922-1

Voyer, A. Silve, L.M. Mir, R. Scoretti and C. Poignard. Computation of the temporal evolution and spatial distribution of the electric field within a biological tissue when a pulse voltage is applied to induce electroporation. Bioelectrochemistry, in press

Azan, P.J. Caspers, T.C. Bakker Schut, S. Roy, C. Boutros, C. Mateus, E. Routier, B. Besse, D. Planchard, A. Seck, N. Kamsu Kom, G. Tomasic, S. Koljenović, V. Noordhoek Hegt, A. Celebic, M. Texier, E. Lanoy, A.M.M. Eggermont, A. Paci, C. Robert, G.J. Puppels, L.M. Mir. A Novel Spectroscopically Determined Pharmacodynamic Biomarker for Skin Toxicity in Cancer Patients Treated with Targeted Agents. Cancer Res. In press

Azan, V. Untereiner, C. Gobinet, G. D. Sockalingum, M. Breton, O. Piot and L. M. Mir. Demonstration of Protein Involvement in Living Cell Electropulsation using Confocal Raman Microspectroscopy. Sci. Rep. In press

Leguèbe, M.G. Notarangelo, M. Twarogowska, R. Natalini, C. Poignard. Mathematical model for transport of DNA plasmids from the external medium up to the nucleus by electroporation. In press in Mathematical Biosciences Journal.

A.Guittet, C. Poignard, F. Gibou. A Voronoi Interface Approach to Cell Aggregate Electropermeabilization. Accepted in Jnl Comp. Phys.

Bardet S.M., Carr L., Soueid M., Arnaud-Cormos D., Leveque P., O’Connor R.P. “Multiphoton imaging reveals that a single 10 nanosecond pulsed electric field collapses tumor perfusion in human glioblastoma xenografts”. Scientific Report, 6:34443. Published: 04 October 2016. DOI:  10.1038/srep34443

Casciola, M.; Tarek, M. A molecular insight into the electro-transfer of small molecules through electropores driven by electric fields. Biochim. Biophys. Acta. Biomembranes, 1858: 2278–2289, 2016

Pillet F; Marjanovič I; Reberšek M; Miklavčič D; Rols MP; Kotnik T. Inactivation of spores by electric arcs. Publisher: BMC Microbiol. In Press; DOI: 10.1186/s12866-016-0764-x

Phez E; Gibot L; Rols MP. How transient alterations of organelles in mammalian cells submitted to electric field may explain some aspects of gene electrotransfer process. Bioelectrochemistry, in press.

Till U; Gibot L; Mingotaud AF; Ehrhart J; Wasungu L; Mingotaud C; Souchard JP; Poinso A; Rols MP; Violleau F; Vicendo P. Drug Release by Direct Jump from Poly(ethylene-glycol-b-ε-caprolactone) Nano-Vector to Cell Membrane. Molecules, in press.

Kulbacka J; Pucek A; Kotulska M; Dubińska-Magiera M; Rossowska J; Rols MP; Wilk KA. Electroporation and lipid nanoparticles with cyanine IR-780 and flavonoids as efficient vectors to enhanced drug delivery in colon cancer. Bioelectrochemistry, DOI: 10.1016/j.bioelechem.2016.02.013

Luengo E; Martínez JM; Coustets M; Álvarez I; Teissié J; Rols MP; Raso J. A Comparative Study on the Effects of Millisecond- and Microsecond-Pulsed Electric Field Treatments on the Permeabilization and Extraction of Pigments from Chlorella vulgaris. J. Membr. Biol. DOI: 10.1007/s00232-015-9796-7

Josserand V; Kéramidas M; Lavaud J; Righini C; Vollaire J; Bellard E; Rols MP; Teissié J; Coll JL; Golzio M. Electrochemotherapy guided by intraoperative fluorescence imaging for the treatment of inoperable peritoneal micro-metastases. J Control Release Subsets: In Press; MEDLINE DOI: 10.1016/j.jconrel.2016.05.003

Pasquet L; Bellard E; Golzio M; Rols MP; Teissie J. A double-pulse approach for electrotransfection. J. Membr. Biol. DOI: 10.1007/s00232-014-9720-6

Escoffre JM; Hubert M; Teissié J; Rols MP; Favard C. Evidence for electro-induced membrane defects assessed by lateral mobility measurement of a GPi anchored protein. Eur. Biophys. J. DOI: 10.1007/s00249-014-0961-1

Rosazza C; Deschout H; Buntz A; Braeckmans K; Rols MP; Zumbusch A. Endocytosis and Endosomal Trafficking of DNA After Gene Electrotransfer In Vitro. Mol Ther Nucleic Acids DOI: 10.1038/mtna.2015.59

Rosazza C; Buntz A; Rieß T; Wöll D; Zumbusch A; Rols MP. Intracellular tracking of single-plasmid DNA particles after delivery by electroporation. Mol. Ther. DOI: 10.1038/mt.2013.182

Batista Napotnik T, Reberšek M, Vernier PT, Mali B, Miklavčič D. Effects of high voltage nanosecond electric pulses on eukaryotic cells (in vitro): A systematic review.Bioelectrochemistry 110: 1-12, 2016.

Čorović S, Mahnič-Kalamiza S, Miklavčič D. Education on electrical phenomena involved in electroporation‑based therapies and treatments: a blended learning approach. Biomed. Eng. Online 15: 36, 2016.

Dermol J, Pakhomova ON, Pakhomov AG, Miklavčič D. Cell electrosensitization exists only in certain electroporation buffers. PLOS One 11(7): e159434, 2016.

Dovgan, B, Barlič, A, Knežević, M, Miklavčič, D. Cryopreservation of Human Adipose-Derived Stem Cells in Combination with Trehalose and Reversible Electroporation. Journal of Membrane Biology: 2016.

Forjanič T., Miklavčič D. Mathematical model of tumor volume dynamics in mice treated with electrochemotherapy. Med. Biol. Eng. Comput., pp. 1–12, Sep. 2016.

Haberl Meglič S, Levičnik E, Luengo E, Raso J, Miklavčič D. The effect of temperature and bacterial growth phase on protein extraction by means of electroporation.Bioelectrochemistry 112: 77-82, 2016.

Kotnik T, Weaver JC. Abiotic gene transfer: rare or rampant? J. Membrane Biol. 249: 623-631, 2016.

Kranjc M, Bajd F, Serša I, de Boevere M, Miklavčič D. Electric field distribution in relation to cell membrane electroporation in potato tuber tissue studied by magnetic resonance techniques. Innov. Food Sci. Emerg. Technol. 37: 384-390, 2016.

Kranjc, M, Bajd, F., Serša, I., Boevere, M. de, Miklavčič, D. Electric field distribution in relation to cell membrane electroporation in potato tuber tissue studied by magnetic resonance techniques. Innovative food science & emerging technologies, ISSN 1466-8564, 2016, vol. , no. , str. 1-31

Kranjc S, Kranjc M, Ščančar J, Jelenc J, Serša G, Miklavčič D. Electrochemotherapy by pulsed electromagnetic field treatment (PEMF) in mouse melanoma B16F10 in vivo. Radiol. Oncol. 50: 39-48, 2016.

Langus, J., Kranjc, M., Kos, B., Šuštar, T., Miklavčič, D. Dynamic finite-element model for efficient modelling of electric currents in electroporated tissue. Scientific reports, ISSN 2045-2322, 2016, 6, pp. 1-11

Maček Lebar A, Velikonja A, Kramar P, Iglič A. Internal configuration and electric potential in planar negatively charged lipid head group region in contact with ionic solution.Bioelectrochemistry 111: 49-56, 2016. 

Pavšelj N, Cuznar P, Veselovskyi S, Miklavčič D, Hart FX. Measuring the viscoelastic properties of tissue using changes in its capacitance: A feasibility study. Elektroteh. Vestn. 83: 99-107, 2016.

Rems L, Miklavčič D. Tutorial: Electroporation of cells in complex materials and tissue. J. Appl. Phys. 119: 201101, 2016.

Sharabi S, Kos B, Last D, Guez D, Daniels D, Harnof S, Mardor Y, Miklavčič D. A statistical model describing combined irreversible electroporation and electroporation-induced blood-brain barrier disruption. Radiol. Oncol. 50: 28-38, 2016.

Stepišnik T, Jarm T, Grošelj A, Edhemović I, Đokić M, Ivanecz A, Trotovšek B, Brecelj E, Potrč S, Čemažar M, Glumac N, Pečnik T, Verberič B, Gadžijev E, Strojan P, Snoj M, Miklavčič D, Serša G .Elektrokemoterapija - učinkovita metoda zdravljenja tumorjev s kombinacijo kemoterapevtika in električnega polja. Zdrav. Vestn. 85: 41-55, 2016. 

Sweeney DC, Reberšek M, Dermol J, Rems L, Miklavčič D, Davalos RV. Quantification of cell membrane permeability induced by monopolar and high-frequency bipolar bursts of electrical pulses. Biochim. Biophys. Acta 1858: 2689-2698, 2016.

Velikonja A, Kramar P, Miklavčič D, Maček Lebar A. Specific electrical capacitance and voltage breakdown as a function of temperature for different planar lipid bilayers. Bioelectrochemistry 112: 132-137, 2016.

Zorec, B., Škrabelj, D., Marinček, M., Miklavčič, D., Pavšelj, N. The effect of pulse duration, power and energy of fractional Er:YAG laser for transdermal delivery of differently sized FITC dextrans. International journal of pharmaceutics, ISSN 0378-5173. [Print ed.], Jan. 2017, vol. 516, no. 1/2, pp. 204-213, http://www.sciencedirect.com/science/article/pii/S0378517316310262, doi: 10.1016/j.ijpharm.2016.10.060.

GROŠELJ, A, KRŽAN, M, KOSJEK, T, BOŠNJAK, M, SERŠA, G, ČEMAŽAR, M. Bleomycin pharmacokinetics of bolus bleomycin dose in elderly cancer patients treated with electrochemotherapy. Cancer chemotherapy and pharmacology, 2016, vol. 77, 939-947, doi: 10.1007/s00280-016-3004-z.

ŠTIMAC, M, KAMENŠEK, U, ČEMAŽAR, M, KRANJC, S, CÖR, A, SERŠA, G. Tumor radiosensitization by gene therapy against endoglin. Cancer gene therapy, 2016, doi: 10.1038/cgt.2016.20.

DOLINŠEK, T, SERŠA, G, PROSEN, L, BOŠNJAK, M, ŠTIMAC, M, RAZBORŠEK, U, ČEMAŽAR, M. Electrotransfer of plasmid DNA encoding an anti-mouse endoglin (CD105) shRNA to B16 melanoma tumors with low and high metastatic potential results in pronounced anti-tumor effects. Cancers, doi: 10.3390/cancers8010003.

BERTINO, G, SERŠA, G, DE TERLIZZI, F, OCCHINI, A, PLASCHKE, CC, GROŠELJ, A, LANGDON, C, GRAU, JJ., MCCAUL, JA., HEUVELING, D, ČEMAŽAR, M, STROJAN, P, DE BREE, R, LEEMANS, C. R, WESSEL, I, GEHL, J, BENAZZO, M. European research on electrochemotherapy in head and neck cancer (EURECA) project : results of the treatment of skin cancer. European Journal of Cancer, 2016, 63, 41-52, doi: 10.1016/j.ejca.2016.05.001.

LAMBRICHT, L, LOPES, A, KOS, Š, SERŠA, G, PRÉAT, V, VANDERMEULEN, G. Clinical potential of electroporation for gene therapy and DNA vaccine delivery. Expert opinion on drug delivery, 2016, vol. 13, 295-310, doi: 10.1517/17425247.2016.1121990.

GRABNER, S, MODEC, B, BUKOVEC, N, BUKOVEC, P, ČEMAŽAR, M, KRANJC, S, SERŠA, G, ŠČANČAR, J. Cytotoxic trans-platinum(II) complex with 3-hydroxymethylpyridine : synthesis, x-ray structure and biological activity evaluation. Journal of inorganic biochemistry, doi: 10.1016/j.jinorgbio.2016.04.031.

PREVC, A, BEDINA ZAVEC, A, ČEMAŽAR, M, KLOBOVES-PREVODNIK, V, ŠTIMAC, M, TODOROVIĆ, V, STROJAN, P, SERŠA, G. Bystander effect induced by electroporation is possibly mediated by microvesicles and dependent on pulse amplitude, repetition frequency and cell type. The journal of membrane biology, 2016, doi: 10.1007/s00232-016-9915-0.

KOS, Š, BLAGUS, T, ČEMAŽAR, M, LAMPREHT TRATAR, U, ŠTIMAC, M, PROSEN, L, DOLINŠEK, T, KAMENŠEK, U, KRANJC, S, STEINSTRAESSER, L, VANDERMEULEN, G, PRÉAT, V, SERŠA, G. Electrotransfer parameters as a tool for controlled and targeted gene expression in skin. Molecular therapy, Nucleic acids, 2016, 5, doi: 10.1038/mtna.2016.65.

PROSEN, L, HUDOKLIN, S, ČEMAŽAR, M, ŠTIMAC, M, LAMPREHT TRATAR, U, OTA, M, ŠČANČAR, J, ROMIH, R, SERŠA, G. Magnetic field contributes to the cellular uptake for effective therapy with magnetofection using plasmid DNA encoding against Mcam in B16F10 melanoma in vivo. Nanomedicine, 2016, 11, 627-641, doi: 10.2217/nnm.16.4.

HRIBERNIK, A, ČEMAŽAR, M, SERŠA, G, BOŠNJAK, M, SNOJ, M. Effectiveness of electrochemotherapy after IFN-[alpha] adjuvant therapy of melanoma patients. Radiology and oncology, 2016, 50, 21-27, doi: 10.1515/raon-2015-0048.

KRANJC, S, KRANJC, M, ŠČANČAR, J, JELENC, J, SERŠA, G, MIKLAVČIČ, D. Electrochemotherapy by pulsed electromagnetic field treatment (PEMF) in mouse melanoma B16F10 in vivo. Radiology and oncology, 2016, 50, 39-48, doi: 10.1515/raon-2016-0014.

DOLINŠEK, T, PROSEN, L, ČEMAŽAR, M, POTOČNIK, T, SERŠA, G. Electrochemotherapy with bleomycin is effective in BRAF mutated melanoma cells and interacts with BRAF inhibitors. Radiology and oncology, 2016, 50, 274-279, doi: 10.1515/raon-2016-0042.

ČEMAŽAR, M, AMBROŽIČ, J, PAVLIN, D, SERŠA, G, POLI, A, KRHAČ LEVAČIĆ, A, TEŠIĆ, N, LAMPREHT TRATAR, U, RAK, M, TOZON, N. Efficacy and safety of electrochemotherapy combined with peritumoral IL-12 gene electrotransfer of canine mast cell tumours. Veterinary and comparative oncology, 2016, doi: 10.1111/vco.12208.

TOZON, N, KRAMARIČ, P, KADUNC KOS, V, SERŠA, G, ČEMAŽAR, M. Electrochemotherapy as a single or adjuvant treatment to surgery of cutaneous sarcoid tumours in horses: a 31-case retrospective study. Veterinary record, 2016, doi: 10.1136/vr.103867.

ŽNIDAR, K, BOŠNJAK, M, ČEMAŽAR, M, HELLER, L C. Cytosolic DNA sensor upregulation accompanies DNA electrotransfer in B16.F10 melanoma cells. Molecular therapy, Nucleic acids, 2016, 5, doi: 10.1038/mtna.2016.34.

 


 

Recent publications

Kotnik, L. Rems, M. Tarek and D. Miklavčič. Membrane Electroporation and Electropermeabilization: Mechanisms and Models. Annu. Rev. Biophys. 48 (2019)

Markelc B, Bellard E, Sersa G, Jesenko T, Pelofy S, Teissié J, Frangez R, Rols MP, Cemazar M, Golzio M. Increased permeability of blood vessels after reversible electroporation is facilitated by alterations in endothelial cell-to-cell junctions. J Control Release. 76:30-41 (2018)

Teissié J, Miklavcic D. Special issue on bacterial inactivation. Bioelectrochemistry. 123:260.  (2018)

Čemažar M, Serša G, Frey W, Miklavčič D, Teissié J. Recommendations and requirements for reporting on applications of electric pulse delivery for electroporation of biological samples. Bioelectrochemistry 122: 69-76 (2018)

Dermol-Černe J, Miklavčič D, Reberšek M, Mekuč P, Bardet SM, Burke R, Arnaud-Cormos D, Leveque P, O'Connor R. Plasma membrane depolarization and permeabilization due to electric pulses in cell lines of different excitability. Bioelectrochemistry 122: 103-114, (2018)

Rems L, Viano M, Kasimova MA, Miklavčič D, Tarek M. The contribution of lipid peroxidation to membrane permeability in electropermeabilization: A molecular dynamics study. Bioelectrochemistry 125: 46-57, (2019)

Pasquet L, Chabot S, Bellard E, Markelc B, Rols, M-P., Reynes J-P, Tiraby G, Couillaud F, Teissié J, Golzio M. Safe and efficient novel approach for non-invasive gene electrotransfer to skin. Scientific reports, 8: 2045-2322 (2018)

 

News

 3rd World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine, and Food & Environmental Technologies

Toulouse, September 3 –6 , 2019

 
Chair
Marie-Pierre Rols

Contact

 LEA EBAM

- Dr Lluis M. MIR, for CNRS, France,

- Pr Damijan MIKLAVCIC, for UL, Slovenia.